hollow-cylindrical polydimethylsiloxane molds (pdms  (Dow Corning)

Journal: Advanced Materials Technologies

Article Title: Collagen‐Tannic Acid Spheroids for β‐Cell Encapsulation Fabricated Using a 3D Bioprinter

doi: 10.1002/admt.202101696

Figure Lengend Snippet: General overview of the collagen hydrogels crosslinked with two different weight fractions of TA 1 wt% (ColTA 1x), and 3 wt% (ColTA 3x) solutions used for this study. a) Chemical structure of TA. TA contains a pentagalloylglucose core in which all hydroxyl functional group are esterified by an additional gallic acid molecule. b) Hydrogen bond interactions between TA molecule and amino acid residues from the collagen peptide chain. c) Fabrication process scheme of the disc‐shaped hydrogels used for the characterization tests. To generate cylinder‐like scaffolds, a PDMS mold was filled with collagen prepolymer solution. Then, the mold was placed at 37 °C. Once the material crosslinks thermally, the scaffolds were submerged in TA solution for 1 min. Scale bar = 10 mm. d) Scanning electron microscope (SEM) images of the hydrogel porosity. First row scale bar = 50 μm. Second row scale bar = 5 μm.

Article Snippet: Collagen hydrogel solution was poured in a cylindrical PDMS (DOW Corning, SYLGARD 184) mold of 10 mm diameter and 3 mm height (Figure ).

Techniques: Functional Assay, Microscopy